Mercury Arc Lamp Fluorescence Microscopy

Commonly used light sources in widefield fluorescence microscopy are light emitting diodes leds mercury or xenon arc lamps or tungsten halogen lamps.

Mercury arc lamp fluorescence microscopy.

Mercury vapour lamps are used to provide a uv light source for epifluorescence microscopy. This accessory has at its upper end the standard royal microscopical society rms thread and can be screwed into the nosepiece. Traditionally almost every research or clinical grade fluorescence microscope has been equipped with a mercury arc lamp. The mercury arc lamp has long been the mainstay light source for fluorescence microscopy because of the bright spectral bands it generates within the visible wavelengths.

Their properties mean they are usually superior in comparison to arc lamps and tungsten halogen lamps which were more commonly used in the past. Mercury and xenon arc lamps are now widely utilized as illumination sources for a large number of investigations in widefield fluorescence microscopy. The most common light sources used today are light emitting diodes leds. Lamp alignment microscope companies may offer an optional centering screen to facilitate the centering of the image of the lamp arc to the back aperture of the objective.

The microscope arc discharge lamp external power supply is usually equipped with a timer to track the number of hours the burner has been in operation. Metal halide bulbs contain more mercury per bulb than mercury arc lamps 0 11g v 0. Focus and alignment of mercury and xenon arc lamps. Discussion of the properties of various fluorescence filter combinations.

Regardless of the pitfalls the mercury arc lamp remains a workhorse in fluorescence microscopy and is still considered one of the best illumination sources especially for low abundance in effect those that have sparse targets or weak fluorophores whose excitation maxima coincide with the spectral lines emitted by the hot mercury plasma. Because the xenon lamp emission profile features a color temperature of approximately 6000 k close to that of sunlight and lacks prominent emission lines this illumination source is more advantageous than mercury arc lamps for many applications in quantitative fluorescence microscopy. Arc lamps lose efficiency and are more likely to shatter if used beyond their rated lifetime 2 00 300 hours. Explore alignment and focusing of the arc lamp in a mercury or xenon burner which simulates how the lamp is adjusted in a real microscope.